Daphnia Reproduction Studies
OECD 211: Daphnia magna Reproduction Test
OCSPP 850.1300 (Draft): Daphnid Chronic Toxicity Test
Studies can be conducted to assess the chronic toxic effect a chemical has on the reproductive capacity of Daphnia magna.
Young daphnids, less than 24 hours old, are exposed to a range of concentrations of the test item for a duration of 21 days. The test can be conducted under semi-static or flow through conditions. A single daphnid is added to each test replicate. The pH, dissolved oxygen and hardness (as CaCO3) are determined.
Immobilisation, behaviour and reproductive capacity are assessed.
Carapace length data for surviving adults is recorded at termination and the test item groups compared to the controls using appropriate statistical methods.
From these assessments the ECx (reproduction), LOEC and NOEC at 21 days can be determined.