Collembolan (Folsomia candida) and Soil predatory mite (Hypoaspis aculeifer) Reproduction Studies
Studies can be performed to determine the effects a chemical has on the reproductive capacity of the Collembolan species, Folsomia candida or the soil predatory mite species, Hypoaspis aculeifer.
The chemical in a range of concentrations is applied to the soil either by incorporation or as a spray to the surface. Age synchronised females are introduced to soil samples and incubated under controlled test conditions for the duration of the study. Moisture content is monitored and adjusted if >2% loss is observed.
A single assessment, made at the end of the study, is conducted to determine the reproductive output, and where possible, the mortality of the original females introduced. Validated methodology is employed to extract the juveniles.
From these data the LC50, ECx, LOEC and NOEC values can be determined.
OECD 226: Predatory mite (Hypoaspis (Geolaelaps) aculeifer) reproduction test in soil
OECD 232: Collembolan Reproduction Test in Soil