can be conducted to determine the effect a chemical has on the parasitic wasp, Aphidius
rhopalosiphi (De Stefani-Perez) (Hymenoptera, Braconidae, Aphidiinae) under
worst case laboratory conditions. This test can be conducted using a glass
plate set up or barley seedlings as a natural substrate.
comprises two assessment phases. During the first phase the wasps are exposed
to the test item as a residue sprayed onto glass plates or barley seedlings,
they are then transferred to aphid infested barley seedlings to assess the
reproductive capacity of the surviving wasps.
first phase, the wasps are transferred to the test unit for exposure to the
fresh dried residues. The test units are then transferred to a controlled
environment and placed in randomised locations.
second phase is to evaluate the reproductive capacity of the surviving wasps. A
single wasp is added to each of test units and the units are incubated for 24
hours to allow for parasitisation. After 24 hours the wasps are removed, and
survival is recorded. The wasps are disposed of and the test units are
incubated for a further 10 – 12 days. At the completion of this phase the
number of mummies on each replicate is determined.
data produced it is possible to determine an LR50 (48 hours) and ERx,
LOER and NOER (fecundity).
Test for evaluating the effects of plant protection products on the parasitic
wasp, Aphidius rhopalosiphi (De Stefani-Perez) (Hymenoptera, Braconidae,
Aphidiinae) Mead-Briggs et al. (2000).
extended laboratory test for evaluating the effects of plant protection
products on the parasitic wasp, Aphidius rhopalosiphi (Hymenoptera,
Braconidae)’ Mead-Briggs et al. (2010).
Non-target Arthropod Studies
of other non-target arthropod studies can also be conducted on the following
- Chrysoperla (Lacewing)
- Orius (Pirate bug)
- Pardosa (wolf spider)
- Poecilus (carabid beetle)
- Trichogramma (polyphagous wasp)